Monocyte Infiltration and c-fms Expression in Hearts of Spontaneously Hypertensive Rats
作者:
Hermann,
Haller Martin,
Behrend Joon Keun,
Park Tom,
Schaberg Friedrich C.,
Luft Armin,
期刊:
Hypertension
(OVID Available online 1995)
卷期:
Volume 25,
issue 1
页码: 132-138
ISSN:0194-911X
年代: 1995
出版商: OVID
数据来源: OVID
摘要:
To elucidate mechanisms of myocardial hypertrophy in spontaneously hypertensive rats (SHR), we examined by Northern blotting the expression of the proto-oncogenes c-myc, c-fos, c-sis, and c-fms in the hearts of 4- and 14-week-old SHR and normotensive Wistar-Kyoto (WKY) rats. No difference in c-myc or c-fos expression could be found between SHR and WKY rats. In SHR, c-sis gave a weak and c-fms a very strong signal at 14 weeks, whereas no signal for these oncogenes was found in either WKY rats or Sprague-Dawley controls. Since c-fms codes for the receptor of monocyte colony-stimulating factor, we next used in situ hybridization to localize the presence of c-fms in hearts of SHR at 14 weeks. We found strong signals for c-fms around small blood vessels and between cardiac myocytes in 14-week-old SHR but none in WKY rats. Immunohistochemical staining corroborated the presence of clusters of monocyte infiltration at these same perivascular sites in significantly greater numbers in SHR than in WKY rats. We conclude that c-fms expression and macrophage infiltration are increased in the perivascular space of hypertrophied hearts from SHR. We suggest that mononuclear cell recruitment and induction of c-fms may play a role in the development of hypertension-associated myocardial hypertrophy. (Hypertension. 1995;25:132-138.)
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