RNA polymerase binding sites on a plasmid R6K derivative with increased copy number
作者:
Jan Nešvera,
Siegfried Klaus,
Jitka Štokrová,
Jitka Hochmannová,
期刊:
Journal of Basic Microbiology
(WILEY Available online 1987)
卷期:
Volume 27,
issue 1
页码: 63-66
ISSN:0233-111X
年代: 1987
DOI:10.1002/jobm.3620270109
出版商: Wiley‐VCH
数据来源: WILEY
摘要:
AbstractThe specific binding ofEscherichia coliRNA polymerase molecules to the DNA of plasmid pNH602, a deletion derivative of R6K having an increased copy number, was detected by electron microscopy. Seven strong RNApol binding sites were found on pNH602 DNA linearized withBamHI orEcoRI restriction endonuclease. All of these specific sites occur in genetically defined regions of the pNH602 molecule. Two of them correspond with the recently reported transcription initiation sites within a region essential for plasmid R6K replication.
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