Retinal pigment epithelial cells produce interleukin-1βand granulocyte-macrophage colony-stimulating factor in response to interleukin-1α
作者:
PlanckStephen R.,
NaXiao,
RobertsonJoseph E.,
RosenbaumJames T.,
期刊:
Current Eye Research
(Taylor Available online 1993)
卷期:
Volume 12,
issue 3
页码: 205-212
ISSN:0271-3683
年代: 1993
DOI:10.3109/02713689308999465
出版商: Taylor&Francis
数据来源: Taylor
摘要:
The retinal pigment epithelium (RPE) is clinically involved in diverse ocular inflammatory diseases. Because perturbed RPE cells produce a variety of inflammatory substances, RPE cells may play an integral part in these diseases. Interleukin-1 (IL-1) and granulocyte-macrophage colony-stimulating factor (GM-CSF) are pleiotropic cytokines with the ability to trigger numerous inflammatory responses. This report shows that cultured human RPE cells synthesize interleukin-1β(IL-1β) and GM-CSF in response to the potentially inflammatory cytokine, IL-1α, but not toE. coliendotoxin. Control RPE cells made little or no mRNA or protein for either IL-1βor GM-CSF. Upon stimulation of the cells by IL-1α, both IL-1βand GM-CSF mRNAs were readily apparent by 3 hours, persisted for over 24 hours, and were translated into immunologically detectable proteins. GM-CSF protein was secreted into the culture medium, whereas IL-1βprotein remained cell associated. The IL-1α-induced mRNA and protein production were inhibited by dexamethasone. These observations provide additional evidence that RPE cells are capable of playing a pivotal role during ocular inflammation.
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