The aim of this study is to present a detailed and thorough desciption of the acetylcholinesterase enzyme (AChE) content of the limbic structures of the rabbit, now commonly used in our laboratory for investigating the electrographic-behavioral correlations of the cholinergic limbic system. We use ‘chemitrodes’ for intracerebral microinjections of cholinomimetic drugs; a detailed histochemical study (using Koelle’s method) of the distribution of AChE enzyme in the limbic system of the rabbit was deemed necessary. Such areas as the septum and the amygdala are particularly poorly described in the rabbit and an attempt was made to clarify the confusion that still exists in the literature on the comparative anatomy and exact localization of these structures. On the whole, it could be inferred from this study that AChE-bearing neurons are generally better developed in phylogenetically older parts of the brain. Practically all the relays in Papez’s ‘cortical circuits of emotion’ have a fairly high AChE activity. As noted by us in other phylogenetically lower macrosmatic mammals, the reaction of AChE in the rabbit brain is more intense than