Characterization of a New Rodent Model of Diabetes insipidus: The Roman High Avoidance Rat Homozygous for Diabetes insipidus
作者:
James P. Herman,
Celia D. Sladek,
Carl T. Hansen,
Don M. Gash,
期刊:
Neuroendocrinology
(Karger Available online 1986)
卷期:
Volume 43,
issue 3
页码: 340-347
ISSN:0028-3835
年代: 1986
DOI:10.1159/000124549
出版商: S. Karger AG
关键词: Brattleboro rat;Diabetes insipidus;Roman high avoidance rat;Roman high avoidance/ diabetes insipidus rat;Vasopressin;Vasopressin deficiency
数据来源: Karger
摘要:
These experiments were designed to characterize the nature and extent of diabetes insipidus present in a new model of genetic vasopressin (VP) deficiency, the Roman high avoidance rat homozygous for diabetes insipidus (RHA: di/di strain). The new strain was developed from an initial cross between Long-Evans derived Brattleboro (LE: di/di) rats and normal Roman high avoidance (RHA: +/+ ) rats, and has been bred to be congenic with the parent RHA: +/+ strain. RHA: di/di rats exhibited polydipsia, excreted dilute urine, and exhibited elevated plasma osmolality. RHA: di/di rats showed a similar urinary response to dehydration as LE: di/di rats. VP was undetectable by radioimmunoassay in the serum, brain, and neurohypophysis of RHA: di/di rats. VP-neurophysin containing cells were not observed in the brains of RHA: di/di rats upon immunocytochemical analysis. Thus, the new RHA: di/di strain exhibits essentially the same profile of diabetes insipidus as the LE: di/di rat. The congenic relationship between RHA: di/di and RHA: +/+ rats makes the RHA: di/di rat a useful model under circumstances where genetic variables unrelated to VP deficiency may confound the interpretation of data.
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