A group of 28 female albino mice, CF-1 strain, 3–6 weeks old, were injected with 1.11mg/kg body weight pyridoxine, and subsequently were restrained for 24 h in individual restraining cages. One half was sacrificed immediately after release and the other half 24 h later. A control group of 28 mice were injected with an equal volume of distilled water and restrained and sacrificed in a similar manner. A third group of 14 untreated mice was also used as control. The pyridoxine groups exhibited an increase in blood glucose levels, in urinary N methylnicotinamide and in brain norepinephrine, while brain serotonin levels remained unchanged. In addition, pyridoxine was found to be protective against ulcers of stomach mucosa. Thus pyridoxine may have an antistress factor as manifested by its regulatory effect on methylation related to production of psychogenic substances such as dimethyloxyphenylethylamine. There is an indication of a possible action of pyridoxine in blocking symptoms of stres