Differentiation Between Endotoxin and Non-Endotoxin Pyrogens in Human Albumin Solutions Using an Ex Vivo Whole Blood Culture Assay
作者:
EdmundJ. Pool,
Gamieda Johaar,
Sandra James,
Ignatius Petersen,
Patrick Bouic,
期刊:
Journal of Immunoassay
(Taylor Available online 1999)
卷期:
Volume 20,
issue 1-2
页码: 79-89
ISSN:0197-1522
年代: 1999
DOI:10.1080/01971529909349315
出版商: Taylor & Francis Group
关键词: pyrogen;IL-6;Polymyxin B;endotoxin;non-endotoxin;pharmaceuticals
数据来源: Taylor
摘要:
Purified E. coli endotoxin, Gram negative bacteria and Gram positive bacteria induce IL-6 secretion by whole blood cultures (WBC's). Polymyxin B at concentrations greater than 2 U/ml completely inhibits IL-6 secretion caused by 10 EU/ml of endotoxin. Polymyxin B has no effect on IL-6 secretion by WBC's in the absence of endotoxin. the inhibition of endotoxin induced IL-6 secretion is Polymyxin B concentration dependent at concentrations less than 1 U/ml. IL-6 induction caused by E. coli is only partially inactivated by 8 U/ml Polymyxin B. Polymyxin B has no effect on IL-6 secretion caused by B. subtilis. Two pyrogenic batches of human serum albumin (HSA), as tested by the rabbit assay for pyrogens, were also investigated. Polymyxin B at 4 U/ml inhibits less than 40 % of IL-6 secretion caused by these pyrogenic HSA batches. All the endotoxin activity in HSA samples spiked with purified endotoxin is inhibited by Polymyxin B indicating that HSA does not protect endotoxin against Polymyxin B inhibition. These results indicate that the pyrogenicity of these HSA batches are caused by Polymyxin B inhibitable and non-inhibitable fractions.
点击下载:
PDF (380KB)
返 回