In Situ Hybridization Using Bromodeoxyuridine Labelled DNA Probe and RNase H
作者:
KitazawaSohei,
KitazawaRiko,
GotohAkinobu,
FujimoriTakahiro,
MaedaSakan,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1993)
卷期:
Volume 68,
issue 3
页码: 137-141
ISSN:1052-0295
年代: 1993
DOI:10.3109/10520299309104683
出版商: Taylor&Francis
关键词: Bromodeoxyuridine;in situ hybridization;RNase H;c-myc;Hodgkin's disease
数据来源: Taylor
摘要:
We developed a modified in situ DNA-RNA hybridization technique and demonstrated c-myc proto-oncogene transcripts in nodular sclerosis type Hodgkin's disease (HD-NS). A hybridization probe was prepared by metabolic labelling of bromodeoxyuridine (BrdU). The hybridized probe was detected with anti-BrdU monoclonal antibody after incubation with ribonuclease H (RNase H), which specifically degrades RNA from DNA-RNA hybrids. In HD-NS, c-myc protooncogene transcripts were demonstrated in the cytoplasm of lacuna cells and a few surrounding lymphoid cells. This modified hybridization method was sensitive and applicable to the study of oncogene expression at a single cell level.
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