The total sporidesmolide fraction from cultures ofPithomyceschartarumcontains at least five cyclodepsipeptides. Four of these are known compounds but the fifth, cyclo-2-oxyisovaleryl-D-alloisoleucyl-D-leucyl-2-oxyisocaproyl-L-valyl-L-N-methylleucyl, appears to be new. Its isolation, physical properties, and structure determination are reported.Keywords:Pithomyceschartarum, sporidesmolide II, sporidesmolide V, sporidesmolic acid D.