Indirect enzyme‐linked Immunosorbent assay for detection of cow's milk in goat's milk
作者:
C. Castro,
R. Martín,
T. García,
E. Rodríguez,
I. González,
B. Sanz,
P. E. Hernández,
期刊:
Food and Agricultural Immunology
(Taylor Available online 1992)
卷期:
Volume 4,
issue 1
页码: 11-18
ISSN:0954-0105
年代: 1992
DOI:10.1080/09540109209354748
出版商: Taylor & Francis Group
关键词: Biotinylated antibodies;enzyme‐linked immunosorbent assay;goat's milk adulteration;goat's milk specific antibodies
数据来源: Taylor
摘要:
An indirect enzyme‐linked immunosorbent assay (ELISA) has been developed for the specific detection of cow's milk (1–25%) in goat's milk. The test uses polyclonal antibodies raised in rabbits against bovine whey proteins (BWP). The anti‐BWP antibodies were recovered from the crude antiserum by immunoadsorption and elution from a column containing immobilized BWP. The anti‐BWP antibodies were biotinylated and rendered cow's milk specific by mixing them with lyophilized ovine and caprine whey proteins. Streptavidin‐peroxidase was used to detect the biotinylated anti‐BWP antibodies bound to bovine milk proteins immobilized on 96‐well plates. The colour developed by the subsequent enzymic conversion of the substrate gave clear absorbance differences when assaying mixtures of goat's milk containing variable amounts of cow's milk.
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