Detection of neurotensin in tissues by capillary zone electrophoresis
作者:
Luc Denoroy,
期刊:
ELECTROPHORESIS
(WILEY Available online 1994)
卷期:
Volume 15,
issue 1
页码: 46-50
ISSN:0173-0835
年代: 1994
DOI:10.1002/elps.1150150108
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractAn electrophoretic method for detecting neurotensin in tissues was developed. Homogenates of rat duodenum and adrenal glands were first extracted by solid‐phase extraction and purified by reversed‐phase high‐performance liquid chromatography. The neurotensin‐rich fraction was further analyzed by capillary zone electrophoresis (CZE) using a commercial instrument with UV detection. A minor peak was detected as neurotensin and its identity was further confirmed by performing several CZE analyses at different pH values. Such an approach could be used to analyze with good molecular specificity the neuropeptides in some human
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