Immunological studies using monoclonal antibodies for detection of low dalton marine toxins
作者:
Yoshitsugi Hokama,
期刊:
Food Additives & Contaminants
(Taylor Available online 1993)
卷期:
Volume 10,
issue 1
页码: 83-95
ISSN:0265-203X
年代: 1993
DOI:10.1080/02652039309374132
出版商: Taylor & Francis Group
关键词: ciguatera;monoclonal anti‐CTX;ciguatoxin;okadaic acid;Stick Enzyme Immunoassay (S‐EIA)
数据来源: Taylor
摘要:
The general methodology employed in the development of an immunological assay system is initially described. These include: (a) the methods and reagents used for coupling low dalton epitopes; (b) selection of appropriate immunogenic carriers; (c) mode of administration and choice of animal; and (d) the method of assessing the antibody produced. The background and methodology, using ciguatoxin as a model, are presented. The stick enzyme immunoassay (S‐EIA) has proven of value to evaluate implicated fish for verification of ciguatera poisoning and for screening of fish associated with ciguatera prior to consumption. No false negatives have occurred with the stick enzyme immunoassay system to date.
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