Autoantibodies to the Ro/Ssa Antigen are Conformation Dependent. I: Anti-60 kD Antibodies are Mainly Directed to the Native Protein; Anti-52 kD Antibodies are Mainly Directed to the Denatured Protein
作者:
ItohYasuhiko,
ReichlinMorris,
期刊:
Autoimmunity
(Taylor Available online 1993)
卷期:
Volume 14,
issue 1
页码: 57-65
ISSN:0891-6934
年代: 1993
DOI:10.3109/08916939309077357
出版商: Taylor&Francis
关键词: Autoantibody;ribonucleoproteins;heterogeneity;systemic lupus erythematosus
数据来源: Taylor
摘要:
Recent studies have shown that Ro/SSA autoantigen is heterogeneous and the autoanti-Ro/SSA response is correspondingly heterogeneous. There are two isoform families; the 60 kD forms and the 52 kD forms.We studied the antigenic difference between the native and denatured Ro/SSA isoforms and found that the autoanti-Ro/SSA response to the native 60 kD antigen is quite homogeneous. All anti-Ro/SSA sera recognize the native kD antigen regardless of the reactivities to the 60 kD band on the Western blot. Surprisingly, no anti-Ro/SSA sera without anti-La/SSB reacts with the native 52 kD Ro/SSA, although sera with both precipitating anti-Ro/SSA and anti-La/SSB can immunoprecipitate the native 52 kD antigen.Anti-Ro/SSA sera exist which react exclusively with the native 60 kD Ro/SSA protein (10/43. 232) while no anti-Ro/SSA sera have been found which react exclusively with the denatured 52 kD Ro/SSA antigen. In sera with anti-Ro/SSA precipitins alone, only antibody to the denatured 52 kD Ro/SSA molecule is found! In sera with anti-Ro/SSA and anti-U1RNP precipitins, no antibody to either native or denatured 52 kD Ro/SSA is found, while in sera with both anti-Ro/SSA and anti-La/SSB precipitins. antibodies to both the native and denatured forms of 52 kD Ro/SSA are present.These data suggest that the anti-Ro/SSA response to the 60 kD molecule is driven by the native 60 kD Ro/SSA molecule while the molecular identification of the antigen drive in the anti-52 kD Ro/SSA response is unknown.
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