The influence of column tubular material on column efficiency in high speed liquid chromatography has been examined. For good reproducibility in HETP, the columns were first packed dry with Corasil I (28–37μ) and then heptane saturated with 3,3′-oxydipropionitrile was flown through the column. When steady state conditions were achieved, the % loading of stationary phase was 1.1%. It was found that column materials of precision bore and seamless stainless steel, aluminum, and copper gave equivalent efficiency results for retained and unretained components. Teflon coated aluminum columns were not reproducible, in regard to HETP.