Isolation of Small Molecular Forms of Factor VIII/von Willebrand Factor from Plasma
作者:
B.A. Perret,
M. Furlan,
E.A. Beck,
期刊:
Pathophysiology of Haemostasis and Thrombosis
(Karger Available online 1984)
卷期:
Volume 14,
issue 4
页码: 289-295
ISSN:1424-8832
年代: 1984
DOI:10.1159/000215077
出版商: S. Karger AG
关键词: Factor VIII/von Willebrand factor;Lower-molecular-weight multimers
数据来源: Karger
摘要:
Cryoprecipitated factor VIII/von Willebrand factor (FVIII/vWF), freed of fibrinogen by clotting with calcium and Defibrase®, was chromatographed on Sepharose CL-2B. Fractions containing lower-molecular-weight forms of FVIII/vWF comprised coprecipitated plasma proteins of similar molecular weights. The major contaminants, fibronectin and IgM, were removed by affinity chromatography on gelatin- and anti-IgM-agarose, respectively. Finally, pure low-molecular-weight FVIII/vWF protein was harvested in the void volume fraction of a Sepharose CL-6B column. The smallest multimers had the size of the tetramer of the basic subunit chain of FVIII/vWF
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