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Determination of phosphate in solution at different ionic composition using malachite green1

 

作者: E. Barberis,   F. Ajmone‐Marsan,   E. Arduino,  

 

期刊: Communications in Soil Science and Plant Analysis  (Taylor Available online 1998)
卷期: Volume 29, issue 9-10  

页码: 1167-1175

 

ISSN:0010-3624

 

年代: 1998

 

DOI:10.1080/00103629809370017

 

出版商: Taylor & Francis Group

 

数据来源: Taylor

 

摘要:

The malachite green method was sometimes used to determine low concentrations of inorganic phosphate due to its high sensitivity. The aim of this work was to test the suitability of this method for the determination of phosphorus (P) extracted by various reagents, e.g., KCl 0.01–1.20M, CaCl20.01–0.1M, Na2SO40.01–0.40M, NaHCO30.1M at pH 8.5, and NaOH 0.1M+NaCl 1M. The malachite green method was also compared with the traditional molybdenum blue method on 35 soil extracts. Color development reached stability within 2 hrs and was stable for up to 24 hrs for dilute solutions. For concentrated solutions the stability was inversely proportional to the concentration of the reagent. Salt concentration appeared to have no effect on absorbance in KCl extracts of up to 1.2M and in Na2SO4extracts of up to 0.05M. Higher concentrations of sodium sulfate induced flocculation and precipitation of the dye complex, as did CaCl2above 0.04 M. A strong correlation was found between the malachite green and the molybdenum blue method. The malachite green method can be used for P determination in soil extracts when appropriate time of color development is provided and salt concentration is taken into account.

 

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