Determination of Cyclosporin A and Metabolites Total Concentration Using a Molecularly Imprinted Polymer Based Radioligand Binding Assay.
作者:
Marion Senholdt2,
Martin Siemann3,
Klaus Mosbach,
LarsI. Andersson1,
期刊:
Analytical Letters
(Taylor Available online 1997)
卷期:
Volume 30,
issue 10
页码: 1809-1821
ISSN:0003-2719
年代: 1997
DOI:10.1080/00032719708001699
出版商: Taylor & Francis Group
关键词: Immunoassay like technique;predetermined selectivity;antibody binding site mimics;radioligand binding assay;polymeric sorbent.
数据来源: Taylor
摘要:
Molecular imprinting of cyclosporin A was undertaken and the resultant polymer preparation was applied to the development of a radioligand binding assay for determination of the total concentration of cyclosporin A and its metabolites. The assay method comprised extraction of haemolyzed whole blood with diisopropyl ether followed by competitive radio-‘immuno’-assay of the organic phase employing imprinted polymer particles and [3H]cyclosporin. The assay measured the parent drug and the metabolites AM1, AM9 and AM4N with equal response. Concomitant analysis of patient samples using a specific EMIT assay in parallel with this novel imprinted polymer-based assay permitted simultaneous determination of the concentration of cyclosporin A and that of its metabolites.
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