Embedding and Fixation Techniques for Immunohistochemical Staining with Anti-DNA Polymeraseαand Ki-67 Monoclonal Antibodies to Analyze the Proliferative Potential of Tumors
作者:
ShibuyaMakoto,
MiwaTetsuro,
HoshinoTakao,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 3
页码: 161-164
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110028
出版商: Taylor&Francis
关键词: fixative;DNA polymeraseα;Ki-67;immunohistochemistry;cell kinetics;nuclear antigens
数据来源: Taylor
摘要:
Anti-DNA polymerase a and Ki-67 are monoclonal antibodies that recognize nuclear antigens expressed in proliferating cells. In this study, we evaluated various methods of embedding and fixing brain tumor specimens to optimize staining with these antibodies. In fresh frozen sections, postfixation with 4% paraformaldehyde, 100% methanol, 95% ethanol and 10% buffered formalin were tested; also tested were prefixation with 4% paraformaldehyde followed by freezing and fixation with 100% methanol, 95% ethanol, or 10% buffered formalin followed by embedding in paraffin. For both antibodies, postfixation of fresh frozen sections with 4% paraformaldehyde at 4 C gave the most intense staining and lowest background activity while preserving histological features. This technique can be used in routine clinical practice to predict the growth potential of tumors.
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