Isolation and characterization of preantral follicles from foetal bovine ovaries
作者:
S. C. J. Hulshof,
J. R. Figueiredo,
J. F Beckers,
M. M. Bevers,
R. van den Hurk,
期刊:
Veterinary Quarterly
(Taylor Available online 1994)
卷期:
Volume 16,
issue 2
页码: 78-80
ISSN:0165-2176
年代: 1994
DOI:10.1080/01652176.1994.9694423
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A simple, mechanical method is described for the isolation of preantral follicles from bovine foetuses of 220–280 days of gestation. On average, 2918 + 621 (s.d.) preantral follicles were isolated per ovary. The isolated preantral follicles were characterized on the basis of the morphological appearance of the surrounding granulosa cells, the number of granulosa cell layers, and their diameter. The results show that primordial, primary, and secondary follicles differ morphologically and that they can be classified by their diameter.
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