The modulation of ecto‐nucleotidase activities by glutamate in cultured cerebellar granule cells
作者:
Carina Boeck,
Mauro Bronzatto,
Diogo Souza,
João Sarkis,
Deusa Vendite,
期刊:
NeuroReport
(OVID Available online 2000)
卷期:
Volume 11,
issue 4
页码: 709-712
ISSN:0959-4965
年代: 2000
出版商: OVID
关键词: Adenosine;Cerebellar granule cells;Ecto-nucleotidase;Glutamate
数据来源: OVID
摘要:
Several enzymes hydrolyze ATP, producing ADP which is hydrolyzed to AMP. Ecto-5′-nucleotidase produces adenosine from AMP. Glutamate (Glu) is an excitatory neurotransmitter and increases extracellular adenosine levels, which is considered an important inhibitory neuromodulator. Here we show that Glu activates ADP and AMP hydrolysis. NMDA and kainic acid (KA) also increased these enzymatic activities, but 1-aminocyclopentane-1S, 3R-dicarboxylic acid (ACPD) had no effect. Dihydrokainate (DHK), an inhibitor of glutamate uptake, also blocked glutamate-evoked activation of ecto-nucleotidases, suggesting that this activation was also Glu transporters dependent. Therefore, we suggest that the Glu-evoked stimulation of ecto-nucleotidases might contribute to the increase of adenosine in extracellular space induced by Glu.
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