Neurosecretion of Human Hypothalamic Immunoreactive β-Endorphin: In vitro Regulation by Dopamine
作者:
Dennis D. Rasmussen,
James H. Liu,
Paul L. Wolf,
Samuel S.C. Yen,
期刊:
Neuroendocrinology
(Karger Available online 1987)
卷期:
Volume 45,
issue 3
页码: 197-200
ISSN:0028-3835
年代: 1987
DOI:10.1159/000124725
出版商: S. Karger AG
关键词: β-Endorphin;Dopamine;Mediobasal hypothalamus;In vitro perifusion
数据来源: Karger
摘要:
An in vitro perifusion system was used to investigate immunoreactive β-endorphin (β-END-I) release from adult human hypothalami in response to dopamine (DA) and the DA receptor antagonist haloperidol (HAL). Administration of a 1 µM pulse of DA consistently elicited a mean (±SE) 88 ± 9% increase (p< 0.05, n = 5) in β-END-I release, whereas 1 µM HAL had no effect. Administration of 1 µM DA during three perifusions in which 1 µM HALwas added to the medium failed to alter basal β-END-I release. In contrast, DA did evoke an acute 230 + 31% increase (p< 0.05) in β-END-I release during three matching perifusions with medium containing the α-adrenergic antagonist phentolamine. These studies demonstrate that DA can stimulate in vitro release of β-END-I from the adult human hypothalamus by a DA receptor mediat
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