The development of a simple, indirect, competitive ELISA is described for the detection and quantification of aldrin/dieldrin in Egyptian milk samples obtained from farm animals of different species used in dairy production in Egypt (buffalo, cow, goat and sheep) and from donkey milk. Milk samples (53) were collected from 18 stations in 16 different governorates in Egypt. The detection limit for aldrin/dieldrin in milk was 10 ppb—5 ppm without enhancement. Aldrin/dieldrin was detected in the following number of milk samples: buffalo, 62.5% (10/16); cow, 73.33% (11/15); goat, 66.66% (8/12); sheep, 71.428% (5/7); and donkey, 66.66% (2/3). The values reflect the lipophilic nature of these pesticides. The assay is rapid, sensitive, simple to perform and excellent for screening.