The authors present a simple liquid chromatographic method which enables direct separation of S(+) and R(−) oxazepam glucuronides and free oxazepam as well as diazepam (internal standard) in a single run. Metabolites and oxazepam are quantified by direct injection of diluted urine. Application to the study of urinary excretion in sheep revealed a S/R ratio of 6.37 which is one of the highest ever observed among various animal species. The percentage eliminated as refered to administrated oxazepam was found to be near 35% which is particularly low compared to other species (60 to 80%).