Effects of preparation techniques on pollen grains ofCentaurea weldeniana(Asteraceae)
作者:
TizianaCusma Velari,
期刊:
Grana
(Taylor Available online 1984)
卷期:
Volume 23,
issue 2
页码: 91-95
ISSN:0017-3134
年代: 1984
DOI:10.1080/00173138409428883
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Two preparation techniques for light microscopy and five for scanning electron microscopy were compared. The most satisfactory method, both from a quantitative point of view, yielding the least variable results, and from a qualitative one, i.e. without causing any distortion of the pollen grains nor of exine structure and sculpture, was critical point drying with dehydration through acetone. On the other hand, it must be emphasized that the coating and acetolysis of the specimens induced quantitative and qualitative alterations.
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