Immunomagnetic T-Lymphocyte Depletion (ITLD) of Rat Bone Marrow Using OX-19 Monoclonal Antibody
作者:
HoffmanA.L.,
MakowkaL.,
CramerD.V.,
CaiX.,
PascualoneA.,
BannerB.F.,
SheahanD.G.,
RettingerP.,
StarzlT.E.,
期刊:
Journal of Investigative Surgery
(Taylor Available online 1989)
卷期:
Volume 2,
issue 3
页码: 241-251
ISSN:0894-1939
年代: 1989
DOI:10.3109/08941938909057430
出版商: Taylor&Francis
关键词: Immunomagnetic T-lymphocyte depletion;bone marrow;allogeneic transplantation;OX-19 monoclonal antibody;graft-versus-host disease
数据来源: Taylor
摘要:
Graft versus host disease (GVHD) may be abrogated and host survival prolonged by in vitro depletion of T lymphocytes from bone marrow (BM) prior to allotransplantation. Using a mouse anti-rat pan T-lymphocyte monoclonal antibody (0×19) bound to monosized, magnetic, polymer beads, T lymphocytes were removed in vitro from normal bone marrow. The removal of the T lymphocytes was confirmed by flow cytometry. Injection of the T-lymphocyte-depleted bone marrow into fully allogeneic rats prevents the induction of GVHD and prolongs host survival.A highly efficient technique of T-lymphocyte depletion using rat bone marrow is described. It involves the binding of OX-19, a MoAb directed against all rat thy-mocytes and mature peripheral T lymphocytes, to monosized, magnetic polymer spheres. Magnetic separation of T lymphocytes after mixing the allogeneic bone marrow with the bead/OX-19 complex provides for a simple, rapid depletion of T lymphocytes from the bone marrow. In vitro studies using flow cytometry and the prevention of GVHD in a fully allogeneic rat bone marrow model have been used to demonstrate the effectiveness of the depletion procedure.
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