Characterisation of a labile RNA virus‐like agent from white clover
作者:
T J GARDINER,
M N PEARSON,
D H HOPCROFT,
R L S FORSTER,
期刊:
Annals of Applied Biology
(WILEY Available online 1995)
卷期:
Volume 126,
issue 1
页码: 91-104
ISSN:0003-4746
年代: 1995
DOI:10.1111/j.1744-7348.1995.tb05005.x
出版商: Blackwell Publishing Ltd
关键词: Virus‐like agent;RNA;white clover
数据来源: WILEY
摘要:
SummaryA labile virus has been identified in white clover in New Zealand. The virus was mechanically transmitted to nine species of herbaceous test plants. No virus‐like particles were identified by electron microscopy in ultrathin sections or in negatively stained sap extracts, although in infectedChenopodium quinoathere were prominent membraneous inclusion bodies in the cell cytoplasm and membrane‐bound structures c. 50 nm in diameter associated with the tonoplast in cell vacuoles. Double‐stranded RNA species of approximately 6800, 3500 and 3300 bp were isolated from infected tissues. DsRNA denatured by boiling was infectious toC. quinoa, but undenatured dsRNA was not infectious. Total nucleic acid preparations from infected leaves were highly infective without boiling, indicating that most of the infectivity was single‐stranded RNA. Infectivity was recovered in the poly (A)‐faction following oligo (dT)‐cellulose chromatography, indicating that the RNA probably lacks a 3′ tract of poly (A). The labile white clover virus is tentatively named white clover vi
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