Nonsequence-specific Inhibition of Bacterial Luminescence by Phosphorothioate Oligodeoxyribonucleotides
作者:
LINDA A. CHRISEY,
MEHRAN PAZIRANDEH,
HEIDI S. LISS,
期刊:
Antisense Research and Development
(MAL Available online 1995)
卷期:
Volume 5,
issue 4
页码: 261-269
ISSN:1050-5261
年代: 1995
DOI:10.1089/ard.1995.5.261
数据来源: MAL
摘要:
To evaluate the effect of synthetic DNA oligomers on regulation of bacterial genesin vivo, we tested 63 oligomers of variable length and chemistry for their ability to selectively suppress light production in the bioluminescent marine organism,Vibrio fischeri. Phosphodiester, phosphorothioate, and mixed backbone oligomers were designed to beluxgene targeted or nontargeted (negative) controls. Although significant suppression of luminescence was observed, most notably with the phosphorothioate oligomers, there was no correlation between inhibitory activity and oligomer sequence. The phosphorothioate oligomer that was most potent for inhibition of luminescence in bacterial culture had no effect on the activity of purified luciferase. Mechanisms other than sequence-specific inhibition of gene expression or direct interaction with luciferase are discussed.
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