β-Adrenergic Stimulation of Adenosine-3’,5’-Monophosphate (c-AMP) in Primary Cultures of Rat Anterior Pituitary Cell Populations Separated by Unit Gravity Sedimentation
作者:
Luc Swennen,
Myriam Baes,
Carla Schramme,
Carl Denef,
期刊:
Neuroendocrinology
(Karger Available online 1985)
卷期:
Volume 40,
issue 1
页码: 78-83
ISSN:0028-3835
年代: 1985
DOI:10.1159/000124055
出版商: S. Karger AG
关键词: c-AMP;Anterior pituitary;β-Receptors;Growth hormone;Prolactin;Cell cultures;Cell separation
数据来源: Karger
摘要:
The effect of the β-adrenergic agonist isoproterenol (ISO) on c-AMP accumulation and on growth hormone (GH) and prolactin (PRL) release was studied in primary cultures of anterior pituitary cell populations with different proportional number of somatotrophs and lactotrophs, obtained by velocity sedimentation at unit gravity. ISO stimulated c-AMP levels in highly-enriched lactotroph (∼7O%) as well as in highly-enriched somatotroph (∼65%) populations. ISO-stimulated c-AMP accumulation was attenuated by dopamine (DA) in highly enriched lactotroph but not somatotroph populations. In a small-cell population consisting of a much lower proportional number of lactotrophs and somatotrophs, the proportional increase of c-AMP accumulation was several times higher than in the other populations and the effect was not suppressible by DA. In this small-cell population, GH but not PRL release was more responsive to ISO than in the other populations. The present observations are consistent with the interpretation that the changes in c-AMP levels induced by ISO originate in part in somatotrophs and lactotrophs. However, the present data also demonstrate that the most responsive cell population in terms of c-AMP accumulation consists of small cells. The majority of these cells could not be identified. The possibility that these cells may be nonsecreting folliculostellate cells is discu
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