Mass measurement accuracy of matrix‐assisted laser desorbed biomolecules: A Fourier‐transform ion cyclotron resonance mass spectrometry study
作者:
Touradj Solouki,
Kent J. Gillig,
David H. Russell,
期刊:
Rapid Communications in Mass Spectrometry
(WILEY Available online 1994)
卷期:
Volume 8,
issue 1
页码: 26-31
ISSN:0951-4198
年代: 1994
DOI:10.1002/rcm.1290080106
出版商: Heyden&Son Limited
数据来源: WILEY
摘要:
AbstractThe use of binary matrices and internal calibrants to improve the mass measurement accuracy in matrix‐assisted laser desorption ionization (MALDI) with Fourier‐transform ion cyclotron resonance (FTICR) mass spectro‐metry is described. Binary matrices enhance the analyte ion yield and enable a complete MALDI‐FTICR mass spectrum to be obtained from a single laser shot. The advantage of single‐laser‐shot data acquisition is that it eliminates line‐broadening due to shot‐to‐shot frequency variations. It is shown that unresolved product ions, mainly due to loss of H2O and/or NH3, shift the centroid of an unresolved multi‐component peak. A mass measurement accuracy of 12 ppm was obtained for the bovine insulin [M+H]+ion using melittin as an
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