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2,2,6,6-Tetramethyl-4-Amino[d17]Piperidine-15N-1-OXYL as a Spin Label For Cell Surface Carbohydrates

 

作者: BennettT. Farmer,   D.Allan Butterfield,  

 

期刊: Analytical Letters  （Taylor Available online 1985）
卷期: Volume 18, issue 5  

页码: 555-561

 

ISSN:0003-2719

 

年代: 1985

 

DOI:10.1080/00032718508066155

 

出版商: Taylor & Francis Group

 

关键词: Perdeuterated;15N-spin labels;enhanced signal-to-noise ratio and increased precision;cell surface sialic acid;electron spin resonance.

 

数据来源: Taylor

 

摘要:

The first use of perdeuterated,15N-Tempamine spin label to selectively monitor cell surface sialic acid of human erythrocyte membranes is reported. The molecular reasons for significantly increased sensitivity and significantly decreased scatter in the data over that of protonated,14N-Tempamine are discussed. These advantages are illustrated by monitoring the effects of wheat-germ agglutinin on the motion of terminal sialic acid of membrane glycoconjugates.

 

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