15-Lipoxygenase Products Stimulate Prolactin Secretion from a Cloned Strain of Rat Pituitary Cells
作者:
Mireille Rabier,
Claude Chavis,
André Crastes de Paulet,
Marcelle Damon,
期刊:
Neuroendocrinology
(Karger Available online 1988)
卷期:
Volume 47,
issue 4
页码: 323-328
ISSN:0028-3835
年代: 1988
DOI:10.1159/000124932
出版商: S. Karger AG
关键词: Lipoxygenase products;Thyrotropin-releasing hormone;Prolactin;GH3 cells
数据来源: Karger
摘要:
Arachidonic acid and its lipoxygenase products may contribute to the process of prolactin (PRL) release. In the present study we investigate the role of 15-hydroperoxyeicosatetraenoicacid (15-HPETE) and 15-hydroxyeicosatetraenoic acid (15-HETE) on PRL secretion from GH3 cells. The incubation of GH3 cells with the lipoxygenase product 15-HETE significantly increased PRL release in a concentration-dependent manner. Nordihydroguaiaretic acid (NDGA), which reduces the production of arachidonate metabolites via the lipoxygenase pathway, also reduced basal and TRH or arachidonic-acid-stimulated-PRL release. The inhibitory effect of NDGA on PRL release could be overcome by the addition of 15-HETE. The time course curve of PRL release from cells challanged by 15-HETE had the same profile as that of cells stimulated by TRH. The stimulating effect of 15-HPETE (ED50 = 0.7 × 10–9M), which is the direct precursor of 15-HETE, on PRL release was greater than TRH or 15-HETE (ED50 M). Furthermore 15-HPETE and 15-HETE seemed to affect the release of newly synthesized PRL. These data indicate that 15-HETE and 15-HPETE could be important intracellular components in the control of PRL secretion and may account for at least a part of arachidonate-induced PRL release from
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