Many practical laser amplifiers exhibit anisotropic gain due to polarization of the pumping fields or to a fixed preferential alignment of the active dipoles. Several specific causes and consequences of gain anisotropy are discussed in detail. In the analysis, the emphasis is placed on dye‐laser systems including arbitrary amplitudes, phases, and polarizations of the pump and signal fields. Analytical results are presented for a unidrectional molecular distribution, and it is found that the polarization states of the pump and signal fields change with distance in the amplifier.