Conformational Analysis of a Complex BetweenDolichos biflorusLectin and the Forssman Pentasaccharide Using Transferred NOE Build-Up Curves
作者:
Meike Rinnbauer,
Emmanuel Mikros,
Thomas Peters,
期刊:
Journal of Carbohydrate Chemistry
(Taylor Available online 1998)
卷期:
Volume 17,
issue 2
页码: 217-230
ISSN:0732-8303
年代: 1998
DOI:10.1080/07328309808002323
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A complex between the Forssman pentasaccharide α-D-GalNAc-(1→3)-β-D-GalNAc-(1→3)-α-D-Gal-(1→4)-β-D-Gal-(1→4)-D-Glc and the seed lectin fromDolichos bifloruswas studied using 2D transfer-NOESY (trNOESY) experiments. Experimental transfer-NOE (trNOE) build-up curves of the complex were compared to theoretical values from a complete relaxation and conformational exchange matrix (CORCEMA) calculation. The dissociation constant and the off-rate for the complex formation were determined by fitting experimental intra-glycosidic trNOE build-up curves to corresponding theoretical curves. Atomic coordinates for the complex were taken from a previous homology-modeling study of a complex between the disaccharide fragment α-D-GalNAc-(1→3)-β-D-GalNAc andD. bifloruslectin. The comparison of experimental and theoretical inter-glycosidic trNOEs expands our previous qualitative NMR study and allows further assessment of the quality of the homology modeling study.
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