AbstractAn optimized high performance liquid chromatographic (HPLC) procedure for the analysis and quantitation of piperidino‐chromone alkaloids has been developed, featuring short retention times, high and adequate resolution. The HPLC system uses a C‐18 reversed phase column eluted with methanol:20 mM potassium dihydrogen phosphate (50:50) with UV detection at λ=254 nm. Comparative studies on the content of chromone alkaloids in the root, stem‐ and root‐bark ofSchumanniophyton magnificumandS. problematicumwere carried out using thi