Preparation of Urea Sensors with a Series Piezoelectric Crystal Device
作者:
Yuanjin Xu,
Changyin Lu*,
Yan Hu,
Lihua Nie,
Shouzhuo Yao,
期刊:
Analytical Letters
(Taylor Available online 1996)
卷期:
Volume 29,
issue 7
页码: 1069-1080
ISSN:0003-2719
年代: 1996
DOI:10.1080/00032719608001459
出版商: Taylor & Francis Group
关键词: biosensor;urease;jack bean tissue;series piezoelectric crystal.
数据来源: Taylor
摘要:
The use of a series piezoelectric crystal (SPC) device for the preparation of biosensors has been investigated. These biosensors are fabricated by mounting the immobilized urease layer or the jack bean tissue slices on the probe surfaces of the SPC ammonia sensor. The urease sensor exhibits favourable frequency response to 1 x 10−5-3x10−3M urea with a response time of ca.3 min. Dynamic range, reproducibility, response time and selectivity of the sensors are discussed. Results show the SPC is an attractive alternative internal sensing element to develop biosensing devices. The urease sensor has been used successfully for the determination of urea in serum samples; the results are comparable to those from a spectrophotometric method.
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