Mathematical simulation of steady state isoelectric focusing of proteins using carrier ampholytes
作者:
Kazuo Shimao,
期刊:
ELECTROPHORESIS
(WILEY Available online 1987)
卷期:
Volume 8,
issue 1
页码: 14-19
ISSN:0173-0835
年代: 1987
DOI:10.1002/elps.1150080104
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractSteady state isoelectric focusing was treated as zero velocity isotachophoresis. From this standpoint, the author's theory of isotachophoresis was modified to deal with isoelectric focusing of protein molecules using carrier ampholytes, and mathematic simulations according to the modified theory were performed to calculate pH, voltag and concentration profiles in steady state isoelectric focusing. The results were in agreement with experiments with regard to resolving power for protein separation.
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