Spectrophotometric Determination of Hesperidin by Ai(III)-Hesperidin Complex in Water-Methanol Solution
作者:
D. Malešev,
Z. Radović,
V. Kuntić,
M. Kosanić,
期刊:
Analytical Letters
(Taylor Available online 1997)
卷期:
Volume 30,
issue 5
页码: 917-926
ISSN:0003-2719
年代: 1997
DOI:10.1080/00032719708002306
出版商: Taylor & Francis Group
关键词: Spectrophotometric methods;pH-metry;Hesperidin;Aluminiumnitrate
数据来源: Taylor
摘要:
The composition and the stability constant of the Al(III)-hesperidin complex were determined by suitable spectrophotometric methods and pH measurements. The formation of the complex, whose concentration stability constant log K1ranged from 3.46 at pH=4.0 to 4.54 at pH=5.0, was established. The conditions for the spectrophotometric determination of hesperidin, by means of the complex formation, were investigated. It was found that hesperidin can be determined in the range from 2.50×10−5M to 1.75×10−4M. The application to the determination of hesperidin in orange juice is demonstrated. All investigations were carried out with 70% methanolic solutions at room temperature (20°C), constant values of pH (3.7), and ionic strength (0.01).
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