Electron Microscope and High Resolution Autoradiographic Studies of Megaloblastic Erythropoiesis
作者:
S.N. Wickramasinghe,
Valerie Bush,
期刊:
Acta Haematologica
(Karger Available online 1977)
卷期:
Volume 57,
issue 1
页码: 1-14
ISSN:0001-5792
年代: 1977
DOI:10.1159/000207854
出版商: S. Karger AG
关键词: Autoradiography;DNA synthesis;Electron microscopy;Folate deficiency;Megaloblasts;Protein synthesis;RNA synthesis;Vitamin B12deficiency
数据来源: Karger
摘要:
Bone marrow fragments from 10 patients with a megaloblastic anaemia due to vitamin B12 or folate deficiency were studied by electron microscopy and electron microscope autoradiography. A proportion of the erythroblasts showed ultrastructural abnormalities. Some of the cells containing autophagic vacuoles, large siderosomes, iron-laden mitochondria, irregularly shaped nuclei, membrane-bound nuclear clefts, or incomplete nuclear membranes were found to be capable of DNA, RNA and protein synthesis. Other cells showed advanced degenerative changes such as the distension of the perinuclear space, the clumping of cytoplasmic organelles near the nucleus and a reduction in the electron density and ribosome content of the cytoplasm. Most of these grossly abnormal cells suffered from either a marked depression or an arrest in protein and RNA synthesis, and were presumably destined for phagocytosis by reticulum cells.
点击下载:
PDF
(4121KB)
返 回