Cis‐Acting Sequences Responsive to the rev Gene Product of the Human Immunodeficiency Virus
作者:
Andrew,
Dayton Ernest,
Terwilliger Joseph,
Potz Mark,
Kowalski Joseph,
Sodroski William,
期刊:
Journal of Acquired Immune Deficiency Syndromes
(OVID Available online 1988)
卷期:
Volume 1,
issue 5
页码: 441-452
ISSN:0894-9255
年代: 1988
出版商: OVID
关键词: Key Words;Human immunodeficiency;virus type;1;Trans;activator;gene;Virion;protein;expression;Cis-acting;antirepression;sequence.
数据来源: OVID
摘要:
Expression of high levels of the structural proteins of the human immunodeficiency virus type 1 (HIV-1) requires the presence of two regulatory genes, the trans-activator (tat), and the regulator of virion protein expression (rev, previously called art or trs). The experiments described here show that expression of virion proteins is dependent upon a small region located in the envelope gene called the cis-acting antirepression sequence (CAR). The CAR region of the envelope sequence is both necessary and sufficient for rev-dependent capsid protein expression. The experiments also show that a defect in either rev or CAR results in a dramatic decrease in the accumulation of the genomic and envelope mRNAs and an overproduction of more extensively spliced viral mRNA species.
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