Chemical parameters of soil profiles of four different forest soils were investigated after glyphosate application in a lysimeter experiment lasting 28 months. Generally, there was hardly any effect found on the nutrient status of the soils due to glyphosate treatment. A slight increase in NO3−and a decrease in pH and extractable Na+were detected. The amounts of extractable NH4+and org.‐N, t‐N, extractable K+, Mg2+, Ca2+, Mn2+, Fen+and Aln+, CEC and BS in the soil profiles were not affected by glyphosate application. These results, taking into consideration the extreme treatment of the soils, justify the conclusion that no harmful ecological effects on forest soil chemical status due to glyphosate application are likely.