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Calibration of Quantitative Histochemical Methods: Estimation of Glycogen Content of Muscle Fibers Using the PAS Reaction

 

作者: Van Der LaarseW. J.,   van NoortP.,   DiegenbachP. C.,  

 

期刊: Biotechnic&Histochemistry  （Taylor Available online 1992）
卷期: Volume 67, issue 5  

页码: 303-308

 

ISSN:1052-0295

 

年代: 1992

 

DOI:10.3109/10520299209110039

 

出版商: Taylor&Francis

 

关键词: quantitative histochemistry;glycogen;PAS;muscle;Xenopus laevis

 

数据来源: Taylor

 

摘要:

A fairly simple method for calibrating microdensitometric histochemical assays is described. The method is based on paired biochemical and histochemical assays on single freeze-dried skeletal muscle fibers which differ widely in their properties. As an example, the method is applied to investigate the validity of the periodic acid-Schiff (PAS) reaction for the microdensitometric estimation of glycogen content. Some problems that may interfere with the calibration are discussed.

 

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