Rapid and simple leaf tissue test for nitrate
作者:
G.C.J. Irving,
D. Bouma,
期刊:
Communications in Soil Science and Plant Analysis
(Taylor Available online 1986)
卷期:
Volume 17,
issue 12
页码: 1299-1310
ISSN:0010-3624
年代: 1986
DOI:10.1080/00103628609367791
出版商: Taylor & Francis Group
关键词: plant analysis
数据来源: Taylor
摘要:
A simple and rapid procedure for the determination of nitrate in fresh leaf tissue, suitable for use by relatively untrained operators is presented. The method requires only 4 reagents and can be carried out in less than 20 min from start to finish. A 400 mg sample of fresh leaf tissue is macerated briefly with 5 drops of 10 N sulphuric acid, diluted with 10.0 ml of water and filtered. Nitrate‐N is estimated on an aliquot of the filtered extract by reducing the nitrate to nitrite by shaking with powdered zinc in ammonium hydroxide solution for 3 min. The reduced mixture is allowed to settle (5 min) and an aliquot is withdrawn through a cotton‐wool plug thus removing any particles of the zinc. The filtered aliquot is then reacted with a single colour reagent to yield a pink azo‐dye, the intensity of which is directly proportional to the amount of nitrate.
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