Mutual Contact of Adherent Polymorphonuclear Leukocytes Inhibits Their Generation of Superoxide
作者:
TanigawaToru,
KotakeYashige,
ItanigawaMar,
ReinkeLester A.,
期刊:
Free Radical Research
(Taylor Available online 1995)
卷期:
Volume 22,
issue 4
页码: 361-373
ISSN:1071-5762
年代: 1995
DOI:10.3109/10715769509145648
出版商: Taylor&Francis
关键词: Cell adhesion;Cell density;Hydrogen peroxide;Cell survival;Electron paramagnetic resonance;Spin trapping
数据来源: Taylor
摘要:
Superoxide generation by polymorphonuclear leukocytes (PMNs) in suspension, or adherent to glass or plastic, after stimulation with /V-formylmethionyl-leucyl-phenylalanine or phorbol myristate acetate was measured by cytochromec reduction and spin trapping. Amounts of superoxide generated by adherent PM Ns were inversely related to cell density. The generation of hydrogen peroxide was also inhibited at higher cell densities. In contrast to adherent cells, superoxide released by PMNs in suspension linearly increased with respect to cell number over a wider range. Microscopic observation indicated that the number of cells in mutual contact increased rapidly at cell densities higher than 4×104cells/cm2, and inhibition of superoxide became apparent at higher cell densities. Mediators which could be released by PMNs, such as NO and adenosine, were not the cause of inhibition. Thesedatu suggest that mutual contact of PMNs suppresses their generation of superoxide. Survival rates of PMNs after stimulation increased at higher densities, indicating that the mutual contact-induced inhibition of superoxide generation by PMNs may be physiologically relevant at sites of inflammation.
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