A New Immunoassay Separation Technique Using Reversibly Soluble Polymers
作者:
DavidL. Marshall,
期刊:
Analytical Letters
(Taylor Available online 1982)
卷期:
Volume 15,
issue 18
页码: 1457-1465
ISSN:0003-2719
年代: 1982
DOI:10.1080/00032718208065137
出版商: Taylor & Francis Group
关键词: Enzyme immunoassay;Sodium alginate;theophylline;alkaline phosphatase
数据来源: Taylor
摘要:
A new immunoassay separation technique was developed based on the use of antibody-polymer conjugates with reversible solubility characteristics. Antibody was coupled to sodium alginate with water-soluble carbodiimide. These conjugates could be used in place of unmodified antibody in various assay formats and removed from solution when desired by converting the polymer to an insoluble form through pH adjustment or addition of metal ions. An enzyme immunoassay for theophylline was used to demonstrate the usefulness of the new approach for both manual and partially automated procedures.
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