Genetic determinants of plasma ACE and renin activity in young normotensive twins
作者:
Gian Rossi,
Krzysztof Narkiewicz,
Maurizio Cesari,
Mikolaj Winnicki,
Justyna Bigda,
Marzena Chrostowska,
Radoslaw Szczech,
Ryszard Pawlowski,
Achille Pessina,
期刊:
Journal of Hypertension
(OVID Available online 1999)
卷期:
Volume 17,
issue 5
页码: 647-655
ISSN:0263-6352
年代: 1999
出版商: OVID
关键词: angiotensin I-converting enzyme;gene polymorphism;kininase II;plasma renin activity;twins
数据来源: OVID
摘要:
ObjectiveWe investigated the determinants of plasma renin activity (PRA) and plasma levels of angiotensin-coverting enzyme (pACE), including the effect of the D/I polymorphism of the angiotensin-converting enzyme (ACE) gene, in monozygotic (MZ) and dizygotic (DZ) twins.MethodsSixty-nine pairs of twins underwent measurements of blood pressure, pACE and ACE D/I genotyping. In addition, in 30 pairs ambulatory blood pressure (ABP) monitoring was carried out. To ascertain twin's zygosity, some highly discriminating variable number of tandem repeats micro- and mini-satellite systems were analysed by polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis and silver staining. The D/I polymorphism was assessed by PCR; pACE was measured in triplicate with a colorimetric assay, and PRA by a commercial kit. In DZ twins, identity by descent of the D/I alleles was examined by PCR amplification of a highly polymorphic simple sequence repeat at the human growth hormone gene.ResultspACE levels were significantly (P< 0.01) higher in DD (9.27 ± 2.60 IU/I, mean ± SD) than in II (6.68 ± 3.0), with DI having intermediate levels (7.93 ± 2.7). No difference of PRA between different D/I genotypes was found. Twin data analysis showed a statistically significant heritability of pACE, but not of PRA. No differences between MZ and DZ twins in PRA, pACE and the relationship of the D/I genotype with pACE was found. Besides showing that the D/I genotype was the most important predictor of pACE, a multivariate analysis demonstrated that identity by descent of the D/I allele, as assessed by growth hormone (GH) genotyping, also significantly affected pACE.ConclusionsIn this study of normotensive twins, pACE and not PRA showed significant heritability, the former being tightly associated with the D/l ACE gene polymorphism, and/or with a quantitative trait locus in linkage disequilibrium with it.
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