Maintenance of Platelet Viability after Platelet-Labeling with Fluorescein Isothiocyanate
作者:
J. Klaverkamp,
K.-P. Völkl,
期刊:
Pathophysiology of Haemostasis and Thrombosis
(Karger Available online 1984)
卷期:
Volume 14,
issue 4
页码: 337-346
ISSN:1424-8832
年代: 1984
DOI:10.1159/000215085
出版商: S. Karger AG
关键词: Blood platelets;Fluorescent labeling;Fluorescein isothiocyanate;ADP aggregation;Ristocetin agglutination;Platelet recovery;Clot elasticity;Release reaction
数据来源: Karger
摘要:
Possible effects of an ex vivo fluorescent labeling procedure of blood platelets on the platelet viability were examined by different function tests. The degree of the impairment of the platelet function proved to be principally correlated with the dye concentration used during the labeling procedure. However, the physiological properties of the labeled platelets obviously remain unrestricted by using intraplatelet fluorescein isothiocyanate (FITC) concentrations below 0.55 mg/1011 platelets, corresponding to 8.5 × 106 FITC molecules bound to one platelet. A procedure for fluorescent labeling of blood platelets is described which is considered to be preferably suitable to prepare platelets for vital microscopic platelet observations
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