Ultrastructural Detection of RNA in Leukemic Cells by the RNase-Gold Method
作者:
IshikawaKohji,
EguchiMitsuoki,
期刊:
Ultrastructural Pathology
(Taylor Available online 1989)
卷期:
Volume 13,
issue 4
页码: 405-412
ISSN:0191-3123
年代: 1989
DOI:10.3109/01913128909048491
出版商: Taylor&Francis
关键词: leukemic cell;RNA;RNase-gold method;electron microscopy
数据来源: Taylor
摘要:
We analyzed semiquantitatively the ultrastructural distribution of RNA by the RNase-gold method in 40 patients with acute leukemia (20 patients with AML and 20 with ALL) before the initial treatment. The number of gold particles showing the presence of RNA was high in the granular component of the nucleolus and cytoplasm but low in the fibrillar component of the nucleolus, granules, the Golgi area, and Auer bodies. The number of gold particles in the nucleolus, nucleus, or cytoplasm was higher in AML than in ALL (p<0.01). The RNase-gold method seems to be useful for evaluating the capacity of protein synthesis, maturity, or differentiation of leukemic cells.
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