Histochemical and Immunological Analyses of Differentiating Skeletal Muscle Fibers of the Postnatal Rat
作者:
Y. Umezu,
K. Hachisuka,
H. Ueda,
M. Yoshizuka,
H Ogata,
S. Fujimoto,
期刊:
Cells Tissues Organs
(Karger Available online 1992)
卷期:
Volume 143,
issue 1
页码: 1-6
ISSN:1422-6405
年代: 1992
DOI:10.1159/000147221
出版商: S. Karger AG
关键词: Myosin ATPase;Western blotting;Differentiating muscle
数据来源: Karger
摘要:
Fiber type differentiation as revealed by myosin ATPase activities and the immunological analysis of myosin light chains (MLC) by the Western blotting method were done on the postnatal rat soleus and extensor digitorum longus (EDL) muscles. In the soleus, type 1 (slow-twitch) muscle fibers gradually increase in percentage after postnatal day 10, and, in the EDL, the differentiation of type 2A and 2B (fast-twitch) muscle fibers is also pronounced between postnatal days 10 and 15. The present immunological analysis of MLC indicates that the antigenicity of LclS becomes more intense with a rapid decrease in anti-genicity of Lc2F from postnatal day 7 in the soleus, while the antigenicity of LclF becomes noticeable during the same period in the EDL. These results suggest that the differentiation of slow-twitch fibers is mainly due to that of Lc1S in the soleus and the differentiation of fast-twitch fibers is mainly due to that of Lc1F in the EDL
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