Inorganic pyrophosphatase in isolated enamel organ and odontoblasts from the rat incisor
作者:
FredénHåkan,
LindeAnders,
MagnussonBengt C.,
期刊:
Acta Odontologica Scandinavica
(Taylor Available online 1975)
卷期:
Volume 33,
issue 3
页码: 143-148
ISSN:0001-6357
年代: 1975
DOI:10.3109/00016357509026355
出版商: Taylor&Francis
关键词: Pyrophosphatase;odontoblasts;amelogenesis
数据来源: Taylor
摘要:
The inorganic pyrophosphatase (PPiase) activity was determined by a colorimetric method in the odontoblasts and the parts of the enamel organ related to enamel matrix formation and enamel maturation. The effects on PPi hydrolysis by EDTA, R 8231, urea and heat treatment were found to be almost identical to those reported for nonspecific alkaline phosphatase (APase) in the same tissues. The Mg2r activation curve for PPiasc was also similar. Like those of APase, these characteristics of PPiase activity were identical in the three locations studied. It is suggested that the close similarity in the properties of PPiase and APase is due to activity of the same enzyme, a concept which is in agreement with recent biochemical and histochemical studies of calcification.
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