Human comeal epithelial water insoluble proteins were used to irtmunize mice. Ircraune splenocytes were fused with Sp 2/0-Ag14 mouse myeloma cells by 50% PEG. Hybridoma antibodies obtained by somatic cell fusion were tested by radioimmunoassay. Supernatants from antibody positive hybrid colonies were used in immuno-fluorescence and crossreaction assays to locate and characterize corneal epithelial antigens. At least three distinct epithelial cell antigens were detected, one of which cross-reacts with rabbit comeal epithelial cells.